Shared_Object_
AMPAR_CaMKII_
strong_coupling
Pathway No. 271Network | 0 | 0.09 | No | - | | 6 | CaMKII | CaMKII
Pathway No. 272 | Network | 20 | 0.09 | No | - |
| | Huge conc of CaMKII. In PSD it is 20-40% of protein, so we assume it is around 2.5% of protein in spine as a whole. This level is so high it is unlikely to matter much if we are off a bit. This comes to about 70 uM. |
| 7 | CaMKII-CaM | CaMKII
Pathway No. 272 | Network | 0 | 0.09 | No | - |
| 8 | CaMKII-thr286*-C
aM | CaMKII
Pathway No. 272 | Network | 0 | 0.09 | No | - |
| | From Hanson and Schulman, the thr286 is responsible for autonomous activation of CaMKII. |
| 9 | CaMKII*** | CaMKII
Pathway No. 272 | Network | 0 | 0.09 | No | - |
| | From Hanson and Schulman, the CaMKII does a lot of autophosphorylation just after the CaM is released. This prevents further CaM binding and renders the enzyme quite independent of Ca. |
| 10 | CaMKII-thr286 | CaMKII
Pathway No. 272 | Network | 0 | 0.09 | No | - |
| | I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off. |
| 11 | CaMK-thr305 | CaMKII
Pathway No. 272 | Network | 0 | 0.09 | No | - |
| | This forms due to basal autophosphorylation, but I think it has to be considered as a pathway even if some CaM is floating around. In either case it will tend to block further binding of CaM, and will not display any enzyme activity. See Hanson and Schulman JBC 267:24 pp17216-17224 1992 |
| 12 | CaM | CaM
Pathway No. 273 | Network | 26.3333 | 0.09 | No | - |
| | There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
| 13 | neurogranin-CaM | CaM
Pathway No. 273 | Network | 0 | 0.09 | No | - |
| 14 | neurogranin* | CaM
Pathway No. 273 | Network | 0 | 0.09 | No | - |
| | The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
| 15 | neurogranin | CaM
Pathway No. 273 | Network | 10 | 0.09 | No | - |
| | Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding .... |
| 16 | CaM-PSD | CaM
Pathway No. 273 | Network | 26.3333 | 0.01 | No | - |
| | There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
| 17 |
neurogranin-CaM_
PSD | CaM
Pathway No. 273 | Network | 0 | 0.01 | No | - |
| 18 | neurogranin_PSD | CaM
Pathway No. 273 | Network | 10 | 0.01 | No | - |
| | Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding .... |
| 19 | neurogranin*_
PSD | CaM
Pathway No. 273 | Network | 0 | 0.01 | No | - |
| | The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
| 20 | CaM-Ca3 | Shared_Object_
AMPAR_CaMKII_
strong_coupling
Pathway No. 271Network | 0 | 0.09 | No | - | |
arrow indicates that ordering is done according to ascending or descending order.
and 
color.